Crosslinking Reagents
Thermo Scientific™ LC-SMCC (succinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxy-(6-amidocaproate))
Crosslink amines to sulfhydryls with this long crosslinker composed of NHS-ester and maleimide groups at ends of a stabilizing cyclohexane-containing, 15-atom spacer arm.
Thermo Scientific™ LC-SDA (NHS-LC-Diazirine) (succinimidyl 6-(4,4'-azipentanamido)hexanoate)
Very short to mid-length (3.9 to 13.5A), amine-to-anything crosslinkers; NHS-ester and diazirine reactive groups; photoactivate with UV light (Amax 330 to 370nm).
Thermo Scientific™ NHS-Azide
Activated azido compounds to derivatize proteins or amine surfaces for selective conjugation, detection or purification by phosphine-modified probes.
Thermo Scientific™ EZ-Link™ TFP Ester-PEG12-DBCO
A heterobifunctional crosslinker that contains an amine reactive tetrafluorophenyl (TFP) and a dibenzylcyclooctyne (DBCO) with a water solubilizing long PEG12 spacer in between. Use to label any primary amine containing molecule such as a protein to biomolecule with a distantly attached DBCO group.
Thermo Scientific™ EZ-Link™ Biotin-PEG12-DBCO
EZ-Link Biotin-PEG12-DBCO is a biotinylation reagent that contains a dibenzylcyclooctyne (DBCO) group for copper-free click chemistry attachment to an azide-labeled molecule, enabling the attachment of biotin using a bioorthogonal approach.
Thermo Scientific™ Sulfo-KMUS (N-κ-maleimidoundecanoyl-oxysulfosuccinimide ester)
Crosslink amines to sulfhydryls with this long crosslinker composed of sulfo-NHS-ester and maleimide groups at ends of an aliphatic, 13-atom (16.3A) spacer arm.
Thermo Scientific™ BM(PEG)3 (1,11-bismaleimido-triethyleneglycol)
Crosslink sulfhydryls to sulfhydryls (cysteine residues) with this long, pegylated, water-soluble crosslinker composed of maleimide groups and 3-unit (17.8A) PEG spacer arm.
Thermo Scientific™ PEG12-SPDP (PEGylated, long-chain SPDP crosslinker)
Very long (54.1A) amine-to-sulfhydryl crosslinker; NHS-ester and pyridyldithiol groups at ends of 12-unit polyethylene glycol spacer arm; forms cleavable disulfide bonds; water-soluble.
Thermo Scientific™ TSAT (tris-(succinimidyl)aminotriacetate)
A 3-way, short-arm (4.2A) amine-to-amine crosslinker; tris (succinimidyl) aminotriacetate, composed of three NHS-ester groups on short spacer arms; membrane permeable.
MilliporeSigma™ Calbiochem™ THIOLYTE™ Monobromobimane Reagent, Fluorescent Labeling Compound
Used to label E.coli thioredoxin.
Thermo Scientific™ SMPT (4-succinimidyloxycarbonyl-alpha-methyl-α(2-pyridyldithio)toluene)
Crosslink amines to sulfhydryls with this crosslinker containing NHS-ester and pyridyldithiol groups at the ends of an aromatic, 6-atom (11.2A) spacer arm.
Thermo Scientific™ KMUH (N-κ-maleimidoundecanoic acid hydrazide)
Crosslink sulfhydryls to periodate-treated glycoprotein sugars with this long (19.0A) crosslinker containing maleimide and hydrazide groups.
Thermo Scientific™ BS2G-d0 (bis(sulfosuccinimidyl) glutarate-d0)
Crosslink amines to amines using these heavy (deuterated) and light matched pairs of a 7.7A, NHS-ester crosslinker for mass spectrometry analysis of protein interactions.
MilliporeSigma™ Calbiochem™ SMCC, Water-Soluble
Used for coupling enzymes to antibodies.
Thermo Scientific™ L-Photo-Methionine
Diazirine analogs of leucine and methionine to express proteins in cell culture that will crosslink their protein interactors upon UV-light activation in vivo.